Yharnam,Queen of Pthumerian

The city of Yharnam was named after her.
Queen Yharnam is seen throughout the game, by this order:
Firstly, after defeating Rom, the Vacuous Spider, gazing up at the red moon.
Secondly, in the Nightmare of Mensis, standing outside the building where Mergo's Wet Nurse is fought.
Thirdly, only if the player returns to her after killing the boss. She will bow to the Hunter, before disappearing, as a sign of gratitude for freeing or perhaps avenging her baby.
The red stains on her dress suggests Mergo, her baby, was taken from her before being born. This, along with the fact that she's handcuffed indicates all of this happened against her will. The purpose of this atrocity seems to be making Mergo cry and drawing the Moon close to the world (as seen in the cutscene after defeating Rom) in order to attract and make contact with the Old Ones.
The Bound Widows are similar to Yharnam: they are bound, wield a dagger, and they're capable of paralyzing the player.
During the Chalice Dungeon fight, she is still pregnant with Mergo. It is unknown the implications of this, yet is can be assumed this fight takes place somewhere where time stands still.

When confronted in the Pthumeru Ihyll Chalice Dungeon, her dress is completely white but becomes bloodier and bloodier as the fight progresses. Her hands are initially chained but she breaks free and wields a dagger in combat. When low on health, she will use her blood to empower her dagger, making it resemble a sword.

Perhaps her most disturbing feature is her pregnancy, the child in her womb (Mergo) constantly cries throughout the battle and is even capable of paralyzing the player with its cries. The pregnancy also helps the player. After taking enough damage, Yharnam will make two clones of herself. The true one is the one that is pregnant. However, it is important to kill the clones, as they will be a major nuisance.