Daughters of Aku

Five of the seven members utilize different weapons: katana, twin sai, twin butterfly swords, kanabo and naginata, with Ashi and Aki both using the Kusarigama.

Ashi was dispatched when Jack allowed her to strike him with her kusarigama. Using the weapon against her, Jack and Ashi dangled from the log and Jack managed to kick her mask off. The fight ended with Jack returning to the log and untying the chain, causing Ashi to plummet. She survived and later was reluctantly rescued by Jack in XCV. She then joined him on his quest after being shown the truth and (Because Aku tried to corrupt and control her) she gained powers similar to his and thus she was able to use time-travel to take Jack back to the past and destroy Aku once and for all. However, as a result of Aku's demise in the past Ashi (And the rest of her sisters) ceases to exist along with the timeline in which they were born. In the end, all of the Daughters of Aku ceased to exist, because, without Aku, they were never born, which happened in the original ending in Episode CI.
All of the daughters minus Ashi in Samurai Jack: Battle Through Time get killed from combat. Ashi is the only survivor in the game's ending (Although a fake Ashi is killed by Aku pushing her on Jack's sword, impaling her).

Ali wielded a katana encountered Jack in the Temple. She destroyed his battle axe and after a brief struggle, Jack swiped her weapon and slit her throat with it, but not before she stabbed him with her kunai. Since Jack had traveled to the past to kill Aku, she has been erased from existence. In Samurai Jack: Battle Through Time, her fate is the same, she just doesn't get to stab Jack this time.
Aki wielded a kusarigama. She was killed in a surprise attack by Jack with a spear. She has been erased from existence due to Jack traveling to the past to destroy Aku.
Ami wielded dual sai. She was killed unintentionally when Jack impaled her with a spear that was originally thrown at Ashi. She has been erased from existence due to Jack traveling to the past and killing Aku.
Ari wielded a kanabo. She was killed while charging at Jack, who impaled her with one of her sisters' butterfly swords. She has been erased from existence due to Jack traveling to the past and destroying Aku.
Ani wielded a naginata. She was defeated when Jack dodged her attack, grabbed her by the shoulders and threw her off the log they were fighting on, which resulted in her death. She also died from probably being digested by the Gargantuan creature. But she is erased from existence now due to Jack traveling to the past to kill Aku.
Avi had wielded dual butterfly swords. She charged at Jack and was dispatched with a single punch that broke her neck, causing her to lose consciousness and fall, which ends up killing her. Like her sister, she died from probably being eaten by the Gargantuan creature but now is erased from existence because of the death of Aku in the past.

Ashi (Erased from existence in the show ending) (Real Ashi survives in the alternate game-ending)
Avi (Killed and erased from existence in the show ending) (Killed in the alternate game-ending)
Ami (Killed and erased from existence in the show ending) (Killed in the alternate game-ending)
Aki (Killed and erased from existence in the show ending) (Killed in the alternate game-ending)
Ani (Killed and erased from existence in the show ending) (Killed in the alternate game-ending)
Ali (Killed and erased from existence in the show ending) (Killed in the alternate game-ending)
Ari (Killed and erased from existence in the show ending) (Killed in the alternate game-ending)
Note: Ashi, Avi, Ami and Aki are the only members of the Daughters of Aku to be named. The only member to be identified by her face is Ashi. The other three members are never named and are instead named by fans for identification. It can best be assumed that Ami and Aki are the two members who say Ashi in Episode XCII as their voice actress, Kari Wahlgren, is given credit under Additional Voices. While the members all have their faces modeled in the Samurai Jack: Battle Through Time video game, they are only identified by DaughterA, DaughterB, DaughterC, DaughterD, DaughterE and DaughterF in their file names.

Stealth: They are masters of stealth and camouflage. In the temple where Jack attempted to hide, they were able to beat him at his own game, vanishing into the shadows to ambush the samurai. However, they were not trained nor equipped for snow concealment and Jack was able to outmatch them in a snowy forest.
Trap Masters: They are capable of creating effective traps.
Assassins: All seven sisters are trained to be highly skilled in assassination (Solely to kill Jack).
Weapon Masters: All seven sisters were masters in their own weaponry, mostly relied on their own weapons and use them very effectively against Jack.
Martial Artists: All seven sisters were trained in melee combat. They have become more skilled at fighting than even their trainers as they shown to killed their own trainers during their final training.

Corrupted Form: Being the biological daughters of Aku, it is safe to say that all seven sisters carry Aku's essence and as such they can be controlled and corrupted by Aku, gaining more powerful forms.
Superhuman Strength: The suit increases their physical strength. This was shown when Ashi was strong enough to penetrate metallic objects with a sword and shatter the ground with one kick.
Elasticity: In their corrupted form, the seven sisters can stretch and expand their limbs in any direction. Their limbs can also act like a whip that can easily rip through metal.
Pyrokinesis/Infusion: The seven sisters' bodysuits have flaming eyebrows similar to their father. They can also infuse weaponry, such as a sword, with fire.
360-Degree Neck Rotation: They can rotate their head without snapping their necks.
Shapeshifting/Weapon Creation: They can turn their forearms into blades.
Eye beams: They can fire energy beams from their eyes.
Time Portal Creation: Much like their father, the seven sisters has the ability to create time portals using their vocal cords. For example, Ashi used this ability to send herself and Jack back in time to defeat Aku once and for all.
Hammerspace: The seven sisters' outfits allowed them to store their weapon of choice when not in use.
Ninjutsu: They are trained in the art of ninjutsu, each of the seven skilled with a different weapon.

Supernatural Physical Ability: During training, the seven sisters were able to run on a cliff as if they were running on flat ground. They even managed to engage in a combat and slay the cultists while doing so. They were able to smash Jack's set of armor pieces as though it was made of paper with mere punches and kicks. During the battle with the orc army, Ashi single-handedly broke through their lines by using one of the soldiers as a battering ram and survived the battle with minor injuries. Amazingly, after completely decimating the large army, Ashi still had enough stamina to not only immediately hold her own against her mother in combat, but to defeat her. The seven sisters' training throughout their entire lives has honed their skills. They are very fast and have amazing jumping and parkour skills which, can arguably be seen as on par with, or even better than Jack's. They can run extremely fast, as they are quick enough to dodge arrow and machine-gun fire. Also, the seven sisters were trained to hone their senses to be able to react to dangers, such as archer fire, by instinct. It is possible that their physical ability is somewhat superhuman due to Aku's essence in them, even without having their true power unlocked.

All seven sisters were trained rigorously for all of their lives to become the deadliest and most dangerous assassins in the world, solely to kill Jack. As a result, they are not only trained in the art of assassination but also void of emotion, shown in how they pursued Jack effectively and unflinchingly even after one of their own died, being raised to believe that death is failure in the eyes of Aku. After years of training, the daughters of Aku became very tactical and analytical, as seen when they strategically stripped Samurai Jack of his armor and weapons during their first encounter. The depth of their training and skill is further revealed during their attempts to kill the Samurai, in which they would attack without mercy, striking with fast-paced coordination, trying their best to ensure that Jack wouldn't have any time to respond or recover.

However, despite their training and numbers, they were only able to stand against Jack with surprise on their side; the Samurai finally overpowered them after a lengthy struggle. Furthermore, due to them spending their lives being trained in isolation, they possess little to no knowledge of the outside world, having never seen other animals such as a doe and assuming a stag was Aku's minion because of its antlers. Ashi in particular was intrigued by the outside world, but her mother refused to allow her to be distracted.

While Jack recovered from his wounds, the Daughters broke out of the ruined temple and continued the hunt for Jack, leaving the corpse of their dead sister behind. Following the river to the forest, the sisters spent a moment observing a female deer in the woods, mistaking her mate for one of Aku's minions due to its antlers. After one of the Daughters scared the deer off and it began to snow, Jack revealed his presence to them. He offered them an ultimatum: leave, or face their destiny. When the Daughters refused and rejected his words of wisdom, Jack emerged from his hiding place in the snow and impaled one of them with a wooden spear. Jack then proceeded to dispatch the rest of the daughters until only Ashi remained, hanging from a fallen tree over a valley. Ignoring her threats about Aku, Jack simply unwrapped her kusarigama from his arm, sending her to her presumed death. Unfortunately, the tree finally collapsed from the fight and Jack followed her into the valley below. (Episode XCIV)

The Daughters of Aku were erased from the timeline since Ashi and Jack altered The Future with the defeat of Aku in The Past. (Episode CI)

In Samurai Jack: Battle Through Time, most of the Daughters of Aku get killed in combat by Jack, except Ashi, who is the only survivor and does not fade away from existence.

The seven daughters were birthed by The High Priestess of an all-female Daughters of Aku sometime after she consumed Aku's essence. After birth, the priestess had them raised as assassins to succeed where many others have failed in the mission of killing Jack. They were put through the most brutal of training, designed to sculpt them into the ultimate ninja warriors, such as facing a hulking, muscle-bound opponent when they were mere children. Even more, they have been utterly taught to think that Aku is the true savior of the planet and Jack seeks to destroy all that Aku has created. When their training was complete, they donned their masks and head coverings and set out to fulfill their mission. (Episode XCII)

They eventually found Jack and proceed to lay a trap after he deals with a large Beetle Drone. They manage to destroy or steal most of his equipment and armor but the Samurai is able to escape their grasp briefly using explosives and flee towards an abandoned temple. After a long search, the sisters manage to locate him and engage in a running battle with him throughout the temple. Eventually, one of the Sisters manages to corner and wound Jack, but at the cost of her life, a fact that leaves Jack horrified as he realizes he has been fighting a living human being instead of another machine as he had initially thought them to be. After snapping out of it, Jack then manages to escape them again by recovering the tuning fork blade he took from the assassin Scaramouche from the now dead sister who stole it and used it to seal the temple hallway behind him as he jumped into the river below. (Episode XCIII)

Being septuplets, they all shared very similar physical traits consisting of black hair, narrow eyes and what appeared to be pitch-black latex-like suits. It is later revealed that their suits are in fact solidified tar as a result of being pushed into a hot, tar-like substance from the Pit of Hate by their mother.[1] The only distinguishing trait among them is their varying hairstyles. When out on their mission, they wear white masks with black horns made to resemble Aku's.

The Daughters of Aku were a group of seven Human-demon hybrid women who were born and raised by their mother, The High Priestess, for the sole purpose of killing Samurai Jack. They are from a cult of the same name dedicated to Aku and appear during the events of Season 5. As of Episode XCV, only Ashi was confirmed to be alive. It was confirmed in Episode XCVIII that all the Daughters, minus Ashi, did indeed perish by Jack according to the High Priestess. In Episode C, it was revealed that they were indeed the biological daughters of Aku, as their mother had (somehow) consumed Aku's essence, which led to her becoming pregnant and eventually giving birth to Ashi and her sisters.

After Jack and Ashi time-traveled into The Past shortly after Aku originally hurled Jack into The Future, Jack finally triumphed in destroying Aku. With his demise in the past, Ashi (And the rest of her sisters) are rendered non-existent and thus were never born. In Samurai Jack: Battle Through Time's ending, Ashi is spared from being erased from existence and lives on in the past as Jack's wife and the sole survivor of the Daughters of Aku.