Yuna Amagami

Shirahi Tsuruyama (鶴山 白日, Tsuruyama Shirahi)
Voiced by: Chika Anzai[8]
Uryu's childhood friend from the orphanage who shares his dream of entering Kyoto University's medical school. She has been in love with Uryu ever since the day she first met him at the orphanage, even though Uryuu has never reciprocated at all, and is dismayed by his newfound relationship with the Amagami sisters, wanting to get rid of them and have Uryuu all to herself. However, Uryu eventually rejects her and helps her realize her mistake, which leads to her mending her relationship with the girls and becoming a temporary maiden at the shrine.

Yomiko Tsukigami (月神 読子, Tsukigami Yomiko)
Voiced by: Yui Horie[7]
A senior priestess at the Tsukigami Shrine and a mentor to the Amagami sisters.

Mahiru Anekōji (姉小路 舞昼, Anekōji Mahiru)
Voiced by: Nana Mizuki[8]
A doctor and the dormitory mother at the orphanage where Uryū used to stay at. She was the one who encouraged Uryū to stay at the Amagami Shrine.

Chidori Amagami (甘神 千鳥, Amagami Chidori)
Voiced by: Sōnosuke Hattori (voice comic),[2] Bin Shimada (anime)[7] (Japanese); Kenny Green[4] (English)
The grandfather of the Amagami sisters and the chief priest of Amagami Shrine, who started caring for the sisters after the death of their mother.

Asahi Amagami (甘神 朝姫, Amagami Asahi)
Voiced by: Miyu Tsuji (voice comic),[2] Ayane Sakura (PV1),[5] Ayana Taketatsu (PV2),[6] Shion Wakayama (anime)[3] (Japanese); Abigail Blythe[4] (English)
The youngest sister, who is a member of her school's track-and-field club. She is a mature middle school scholar despite her age. However, she has a complex about her small breasts. She fell in love with Uryū after the incident involving a time loop. It is later revealed that she too was adopted into the Amagami family and that she was born Miyuki Kiyomizu (清水 美雪, Kiyomizu Miyuki).

Yae Amagami (甘神 夜重, Amagami Yae)
Voiced by: Miyu Tsuji (voice comic),[2] Ai Kayano (PV1),[5] Reina Ueda (PV2),[6] Sumire Uesaka (anime)[3] (Japanese); Monica Rial[4] (English)
The eldest daughter and an art major at a university with a playful personality. It is later revealed that she was actually adopted into the Amagami family and that her birth name is Reiko Ichijōji (一乗寺 澪子, Ichijōji Reiko). She has had a crush on Uryū since their short encounter few years ago, even though he does not reciprocate at all. Uryu's encouragement at the time led her to become more decisive and more carefree which allowed her to leave her dark childhood behind which helps shape her current personality.

Uryū Kamihate (上終 瓜生, Kamihate Uryū)
Voiced by: Sōnosuke Hattori (voice comic),[2] Ryōta Suzuki (anime)[3] (Japanese); Branden Loera[4] (English)
A high school student aiming to enter Kyoto University's medical school. He was inspired to become a doctor after his mother's death. Despite being engrossed in studying most of the time, he managed to bond with the Amagami siblings in spite of their rough start and is destined to marry one of the siblings in order to continue the legacy of Amagami Shrine. Because he believes that God did not answer his prayers for his mother to get better, he initially does not believe in spirituality or religion, and hates the idea of relationships and having a significant other. Initially, he has no romantic feelings for any of the girls at all, and refuses to marry any of them, wanting to get away from them and the shrine as soon as possible. However, he eventually warms up to the girls and starts treating them better. He's very capable of doing household chores and cooking which the Amagami siblings adore very much. A frequent running gag of Uryu revolves around him getting caught in awkward situation where the Amagami siblings, Yuna in particular, keep on chiding him for being perverse.

Background: Born Nadeshiko Kurama, she was orphaned following a fire incident before being adopted into the Amagami family.

Role and Interests: As the middle sister, she holds a central role in the household. She is a high school student and has a soft spot for cats.

Relationship with Uryu: Initially, she is the most opposed to Uryu living at the shrine, frequently finding reasons to criticize him. Over time, she develops a deep crush on him, particularly after a body-swapping incident helped her understand him better.

Personality Traits: Serious, responsible, and often "stiff," Yuna takes her duties as a miko very seriously. She is a "tsundere," often appearing harsh, blunt, or easily annoyed (particularly with Uryu) but has a caring, passionate, and warm side.

Yuna Amagami from Tying the Knot with an Amagami Sister is the serious, responsible, and pious second sister who serves as a shrine maiden. She acts as a tsundere, initially hostile to Uryu Kamihate's presence but growing to love him. She is deeply dedicated to protecting the Amagami Shrine and, despite a strict exterior, deeply cares for her family.